Proper Use of Molecular Analysis Techniques for SNPs and Detection of Small Deletions
by Dr Zafar Iqbal PhD Postdoctorate
(KSAU-HS, NGHA, Riyadh, Saudi Arabia)
Overall, it is an interesting article. However, presence of SNP cannot be ruled out using PCR and agarose gel electrophoresis. Allele-specific PCR and sequencing are required to detect SNPs. Moreover, even for small deletions, it is better to validate PCR-based techniques by using DNA sequencing.
Dr Zafar Iqbal
M.Phil., Ph.D. (Molecular Genetics / Hematology & Oncology)
Post-doc. (Funct. Medical Genomics / Clinical Hematology & Oncology)
Member AACR, ASCO (USA) SSBMT (KSA) NAYU (Pak) EHA (EU)
Fellow ASPHO (USA) SIH (Shanghai, PRC)
Assistant Professor (Medical Genetics / Hematology & Oncology)
CAMS, King Saud Bin Abdulaziz University for Health Sciences (KSAU-HS)
National Guard Health Affairs, Mail Code 3129, Riyadh 11481, Saudi Arabia.
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