An Assessment of Genetic Relationship Among Superior Accessions of Labisia Pumila Analized by Amplified Fragment Length Polymorphism (AFLP) Markers

by Open Science Repository Agriculture
(March 2013)

Abstract

Labisia pumila, locally known as Kacip Fatimah, and member of the Myrsinaceae family, is one of the popular herbal species in Malaysia. The leaf or the whole plant is traditionally used to treat internal problems and women’s health. The objective of this study was to evaluate the genetic relationships among superior accessions for two varieties of L. pumila (L. pumila var. pumila and L. pumila var. alata) using amplified fragment length polymorphism (AFLP) technique. Superior accessions of both varieties were selected based on their total phenolic contents, ranging from 2597 to 2928 mg/50 g GAE (gallic acid equivalent), which had been determined during a previous work. Three primer combinations that produced clear scorable and highly polymorphic bands were screened from 64 EcoRI and MseI primer combinations. The numbers of AFLP fragments generated per primer set ranged from 30 to 124 with fragment sizes varying from 50 to 500 bp. A total of 170 AFLP fragments were detected and all are polymorphic. AFLP banding patterns were then transformed into presence–absence binary data and matrices were processed with NTSys software programs. Results showed that the genetic relatedness for L. pumila var. pumila and L. pumila var. alata was not close at J=0.300 and J=0.298, respectively. No close genetic relatedness was also recorded between the two varieties at J= 0.260. Dendogram based on UPGMA method demonstrated that the two varieties fell into different clusters. The important finding of this study is the identification of genetic relationships among the 119 accessions of both varieties with high total phenolic content which can be used for future breeding activities.

Keywords: Labisia pumila var. pumila, L. pumila var. alata, total phenolic content.

Full text

doi: 10.7392/Agriculture.70081945.