In Vitro Levels of Calcium, Phosphate and Alkaline Phosphate Activity in Media of Rat Osteoblasts Grown in the Presence of Various Implant Materials

by Open Science Repository Dentistry
(May 2013)

Abstract

We studied the in vitro levels of calcium, phosphate, and alkaline phosphate (ALP) activity in media of rat osteoblasts grown in the presence of various implant materials. Osteoblast cells from newborn Sprague-Dawley rats were harvested from calvaria. Cells were cultivated in the presence of Cobalt-Chrome (Co-Cr), cpTitanium (Ti), polymethylmethacrylate (PMMA), and wood in an artificial medium in a humidified atmosphere (5% CO2) at 37 oC. Cellular function was monitored over two weeks. Statistical tests (2-way ANOVA) of the results indicated that changing the disc material did not have a significant effect (p>0.05) on the amounts of Ca2+ and PO42- produced but it did on the level of ALP activity in the medium (p<0.05). The culture time impacted significantly (p<0.05) on the levels of all 3 markers. Further, the interaction effect between the disc material and culture time was significant (p<0.05) for all markers. Wood produced the highest activity for ALP. Electron Microscopic studies captured cells growing over all materials, but the presence of Ca2+ was detected in wood samples only. With respect to markers for bone function, wood is comparable to known implant materials.

Keywords: rat osteoblasts, dental Implant materials, calcium/phosphate/ALP levels, ANOVA tests.

Full text

In Vitro Levels of Calcium, Phosphate and Alkaline Phosphate Activity in Media of Rat Osteoblasts Grown in the Presence of Various Implant Materials