Assessment of Digestion Procedure for Determination of Trace Elements by ICP-OES

by Open Science Repository Chemistry
(February 2013)


A simple and reliable analytical procedure for determination of selected trace elements such as Al, B, Ba, Cd, Cr, Cu, Mn, Pb and Zn in human blood samples was modified. Samples were collected from normal subjects, diabetic mellitus and hypertensive patients. Samples were taken from both genders of different ages from occupants of urban populations of Taif city, Saudi Arabia. Different sample preparation procedures with acids and oxidizing reagents were tested and well investigated. Analysis was made by inductively coupled plasma optical emission spectrometry after sample digestions. The overall recoveries of all determined elements were found in the range of (94.6–104.9%) of the expected values. The results of this study showed that the mean concentrations of the Al, B, Ba, Cd, Cu and Pb in human blood of diabetic mellitus and hypertensive patients were higher than the corresponding values of normal subjects. While the concentrations of Cr, Mn and Zn in human blood of diabetic mellitus and hypertensive patients were lower as compared to values of normal subjects, the differences found were non-significant (p=0.05).

Keywords: trace elements, digestion procedure, diabetes mellitus, hypertension, Inductively coupled plasma-optical emission spectrometry (ICP-OES).

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doi: 10.7392/Chemistry.70081933.

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