Open Science Repository Agriculture

doi: 10.7392/openaccess.45011822


Identification of different species of Rhodotorula using Internal Transcribed Spacers 


Mona Abdel-mageed Mohamed, Ashraf Bakry Abdelrazik, Samir Abdel-Aziz Ibrahim

Department of Genetic, Faculty of Agriculture and Ain Shams Centre for Genetic Engineering and Biotechnology, Ain Shams University, Cairo, Egypt


Abstract

The identification and classification of yeasts have traditionally been based on morphological, physiological and molecular identification. In the present study, six yeast isolates were isolated from potatoes and maize all the isolates able to produce carotenoid. Rhodotorula spp. was identified by physiological and molecular genetic tools. Physiological identification were carried out by assimilation of carbon sources, assimilation of nitrogen sources and antibiotic resistance pattern using disk diffusion method. The fragment size polymorphism (FSP) were used for molecular genetic identification. The Internal Transcribed Spacer (ITS1) and (ITS2) regions of the isolates were amplified separately. The isolates were grouped based on fragment size polymorphism (FSP) of the amplicon. For precise and final identification, the ITS-PCR products were subjected to sequencing followed by Blast analysis. As a result, four isolates were identified as Rhodotorula glutinis and two isolates were identified as Rhodotorula mucilaginosa.

Keywords: Rhodotorula, Internal Transcribed Spacers, identification and classification of yeasts, agriculture.



Citation: Mohamed, M. A., Abdelrazik, A. B., & Ibrahim, S. A.-A. (2014). Identification of different species of Rhodotorula using Internal Transcribed Spacers. Open Science Repository Agriculture, Online(open-access), e45011822. doi:10.7392/openaccess.45011822

Received: September 18, 2014

Published: September 22, 2014

Copyright: © 2014 Mohamed, M. A., Abdelrazik, A. B., & Ibrahim, S. A.-A. Creative Commons Attribution 3.0 Unported License.

Contact: research@open-science-repository.com



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APA

Mohamed, M. A., Abdelrazik, A. B., & Ibrahim, S. A.-A. (2014). Identification of different species of Rhodotorula using Internal Transcribed Spacers. Open Science Repository Agriculture, Online(open-access), e45011822. doi:10.7392/openaccess.45011822

MLA

Mohamed, Mona Abdel-mageed, Ashraf Bakry Abdelrazik, and Samir Abdel-Aziz Ibrahim. “Identification of Different Species of Rhodotorula Using Internal Transcribed Spacers.” Open Science Repository Agriculture Online.open-access (2014): e45011822.

Chicago

Mohamed, Mona Abdel-mageed, Ashraf Bakry Abdelrazik, and Samir Abdel-Aziz Ibrahim. “Identification of Different Species of Rhodotorula Using Internal Transcribed Spacers.” Open Science Repository Agriculture Online, no. open-access (September 22, 2014): e45011822. doi:10.7392/openaccess.45011822.

Harvard

Mohamed, M.A., Abdelrazik, A.B. & Ibrahim, S.A.-A., 2014. Identification of different species of Rhodotorula using Internal Transcribed Spacers. Open Science Repository Agriculture, Online(open-access), p.e45011822.

Science

1. M. A. Mohamed, A. B. Abdelrazik, S. A.-A. Ibrahim, Identification of different species of Rhodotorula using Internal Transcribed Spacers, Open Sci. Repos. Agric. Online, e45011822 (2014).

Nature

1. Mohamed, M. A., Abdelrazik, A. B. & Ibrahim, S. A.-A. Identification of different species of Rhodotorula using Internal Transcribed Spacers. Open Sci. Repos. Agric. Online, e45011822 (2014).


doi

Research registered in the DOI resolution system as: 10.7392/openaccess.45011822.


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This work is licensed under a Creative Commons Attribution 3.0 Unported License.