Identification of different species of Rhodotorula using Internal Transcribed Spacers

by Open Science Repository Agriculture
(September 2014)

Abstract

The identification and classification of yeasts have traditionally been based on morphological, physiological and molecular identification. In the present study, six yeast isolates were isolated from potatoes and maize all the isolates able to produce carotenoid. Rhodotorula spp. was identified by physiological and molecular genetic tools. Physiological identification were carried out by assimilation of carbon sources, assimilation of nitrogen sources and antibiotic resistance pattern using disk diffusion method. The fragment size polymorphism (FSP) were used for molecular genetic identification. The Internal Transcribed Spacer (ITS1) and (ITS2) regions of the isolates were amplified separately. The isolates were grouped based on fragment size polymorphism (FSP) of the amplicon. For precise and final identification, the ITS-PCR products were subjected to sequencing followed by Blast analysis. As a result, four isolates were identified as Rhodotorula glutinis and two isolates were identified as Rhodotorula mucilaginosa.

Keywords: Rhodotorula, Internal Transcribed Spacers, identification and classification of yeasts, agriculture.

Full text

Identification of different species of Rhodotorula using Internal Transcribed Spacers