Open Science Repository Dentistry

doi: 10.7392/openaccess.70081966

In Vitro Levels of Calcium, Phosphate and Alkaline Phosphate Activity in Media of Rat Osteoblasts Grown in the Presence of Various Implant Materials

Fiayaz A. Shaama, M.Phil., D.D.S., M.Sc. [1], Shivananda B. Nayak, M.Sc., Ph.D. [2], Valerie A. Stoute,  Ph.D., M.Sc. [3]

[1] Lecturer, Restorative Dentistry,  Prosthetics unit, Faculty of Medical Sciences, the University of the West Indies, EWMSC, Mt Hope, Trinidad and Tobago, [2] Professor, Department of Preclinical Sciences, Biochemistry unit, Faculty of Medical Sciences, the University of the West Indies, EWMSC, Mt Hope, Trinidad and Tobago, [3] Professor, Departments of Environmental and Graduate Studies, the University of Trinidad and Tobago

Abstract

We studied the in vitro levels of calcium, phosphate, and alkaline phosphate (ALP) activity in media of rat osteoblasts grown in the presence of various implant materials. Osteoblast cells from newborn Sprague-Dawley rats were harvested from calvaria. Cells were cultivated in the presence of Cobalt-Chrome (Co-Cr), cpTitanium (Ti), polymethylmethacrylate (PMMA), and wood in an artificial medium in a humidified atmosphere (5% CO₂) at 37 ^oC. Cellular function was monitored over two weeks. Statistical tests (2-way ANOVA) of the results indicated that changing the disc material did not have a significant effect (p>0.05) on the amounts of Ca²⁺ and PO₄^2- produced but it did on the level of ALP activity in the medium (p<0.05). The culture time impacted significantly (p<0.05) on the levels of all 3 markers. Further, the interaction effect between the disc material and culture time was significant (p<0.05) for all markers. Wood produced the highest activity for ALP. Electron Microscopic studies captured cells growing over all materials, but the presence of Ca²⁺ was detected in wood samples only. With respect to markers for bone function, wood is comparable to known implant materials.

Keywords: rat osteoblasts, dental Implant materials, calcium/phosphate/ALP levels, ANOVA  tests.

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Cite this paper

APA

Shaama, F. A., Nayak, S. B., & Stoute, V. A. (2013). In Vitro Levels of Calcium, Phosphate and Alkaline Phosphate Activity in Media of Rat Osteoblasts Grown in the Presence of Various Implant Materials. Open Science Repository Dentistry, Online(open-access), e70081966. doi:10.7392/openaccess.70081966

MLA

Shaama, Fiayaz A., Shivananda B. Nayak, and Valerie A. Stoute. “In Vitro Levels of Calcium, Phosphate and Alkaline Phosphate Activity in Media of Rat Osteoblasts Grown in the Presence of Various Implant Materials.” Open Science Repository Dentistry Online.open-access (2013): e70081966.

Chicago

Shaama, Fiayaz A., Shivananda B. Nayak, and Valerie A. Stoute. 2013. “In Vitro Levels of Calcium, Phosphate and Alkaline Phosphate Activity in Media of Rat Osteoblasts Grown in the Presence of Various Implant Materials.” Open Science Repository Dentistry Online (open-access) (May 27): e70081966. doi:10.7392/openaccess.70081966. http://www.open-science-repository.com/dentistry-70081966.html.

Harvard

Shaama, F.A., Nayak, S.B. & Stoute, V.A., 2013. In Vitro Levels of Calcium, Phosphate and Alkaline Phosphate Activity in Media of Rat Osteoblasts Grown in the Presence of Various Implant Materials. Open Science Repository Dentistry, Online(open-access), p.e70081966. Available at: http://www.open-science-repository.com/dentistry-70081966.html.

Science

1. F. A. Shaama, S. B. Nayak, V. A. Stoute, In Vitro Levels of Calcium, Phosphate and Alkaline Phosphate Activity in Media of Rat Osteoblasts Grown in the Presence of Various Implant Materials, Open Science Repository Dentistry Online, e70081966 (2013).

Nature

1. Shaama, F. A., Nayak, S. B. & Stoute, V. A. In Vitro Levels of Calcium, Phosphate and Alkaline Phosphate Activity in Media of Rat Osteoblasts Grown in the Presence of Various Implant Materials. Open Science Repository Dentistry Online, e70081966 (2013).

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Research registered in the DOI resolution system as: 10.7392/openaccess.70081966.

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